Generate CSS For Sprite Images – Sprite Cow: "
With their increasing popularity, we are seeing more and more CSS sprite generators everyday.

Sprite Cow is another one which is open source and works very effective by auto-recognizing the different elements inside a single image.

So, you design the sprite image in your favorite imaged editing application, upload it to Sprite Cow and simply click (or click + drag) on each element to get their width, height and background-position as a CSS rule.

The application can select items in any size and also gives you the option of changing the background color.